Effects of different concentrations of eugenol in maturation medium on bovine oocytes, oxidative status and preimplantation embryos

Autor:	Leonardo Vitorino Costa de Aquino, Vicente José de Figueirêdo Freitas, Alexsandra Fernandes Pereira, Rodolfo Ungerfeld, Luciana Medeiros Bertini, Lhara Ricarliany Medeiros de Oliveira, Maria Valéria de Oliveira Santos
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Reactive oxygen species Antioxidant medicine.medical_treatment Embryo Glutathione medicine.disease_cause In vitro maturation Eugenol Andrology chemistry.chemical_compound chemistry medicine Animal Science and Zoology Cysteamine Oxidative stress Food Science
Zdroj:	Animal Production Science. 62:142-151
ISSN:	1836-5787 1836-0939
DOI:	10.1071/an21197
Popis:	Context Eugenol is a bioactive compound with defined attributes, including a role in reducing oxidative stress. Therefore, it is an interesting candidate for in vitro embryo production that has an environment that favours the formation of reactive oxygen species. Aim The objective of the study was to evaluate different concentrations of eugenol in bovine oocytes during in vitro maturation to observe the oxidative status and embryonic development. Methods Oocytes were allocated into five groups: control (without antioxidant), 100 μM cysteamine, 83 μM eugenol (E83), 100 μM eugenol and 120 μM eugenol. Three experiments were performed with 23 replicates. Oocytes were evaluated for metaphase II, first polar body, cytoplasmic maturation, cumulus cell expansion and viability. Oxidative status was measured by mitochondrial membrane potential, reactive oxygen species and glutathione. Oocytes were artificially activated and cultured in vitro. Key results The presence of eugenol at 83 μM improved the metaphase II rate compared with all treatments (P
Databáze:	OpenAIRE
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