Regulation of adult neural progenitor cells by Galectin-1/β1 Integrin interaction

Autor: Hirobumi Tada, Jun Hirabayashi, Toshihiko Kadoya, Noboru Uchiyama, Kazunobu Sawamoto, Osamu Yamada, Yoichi Imaizumi, Françoise Poirier, Tetsuro Shingo, Masanori Sakaguchi, Hideyuki Okano, Ko Hayama, Tsuyoshi Morishita, Takuya Shimazaki, Atsushi Kuno
Rok vydání: 2010
Předmět:
Zdroj: Journal of Neurochemistry.
ISSN: 1471-4159
0022-3042
DOI: 10.1111/j.1471-4159.2010.06712.x
Popis: Neural stem cells (NSCs) proliferate and generate new neurons in the adult brain. A carbohydrate-binding protein (lectin), Galectin-1, is expressed in the NSCs in the subependymal zone (SEZ) of the adult mouse brain. The infusion and knockout of Galectin-1 in the SEZ results in an increase and decrease, respectively, of NSCs and subsequently born progenitor cells. The molecular mechanism of this effect, however, has been unknown. Previous studies outside the brain suggest that Galectin-1 binds to a carbohydrate structure of beta1 Integrin and modulates cell adhesion. Here, we studied the functional interaction between Galectin-1 and beta1 Integrin in the adult mouse SEZ. Beta1 Integrin was purified from adult SEZ tissue by binding to a Galectin-1 affinity column, and this binding depended on Galectin-1's carbohydrate-binding activity. In adult brain sections, Galectin-1-binding activity was detected on beta1 Integrin-expressing cells in the SEZ. Furthermore, in the adult SEZ, the simultaneous infusion of a beta1 Integrin-neutralizing antibody with Galectin-1 protein reversed the increasing effect of Galectin-1 on the number of adult neural progenitor cells (NPCs). Finally, intact beta1 Integrin was required for Galectin-1's function in NPC adhesion in vitro. These results suggest that the interaction between beta1 Integrin and Galectin-1 plays an important role in regulating the number of adult NPCs through mechanisms including cell adhesion.
Databáze: OpenAIRE
Externí odkaz: