| Autor: |
Helen H. Benford, James T. Bradley |
| Rok vydání: |
1986 |
| Předmět: |
|
| Zdroj: |
Journal of Insect Physiology. 32:109-116 |
| ISSN: |
0022-1910 |
| DOI: |
10.1016/0022-1910(86)90129-0 |
| Popis: |
The timing of in vivo induction of vitellogenin synthesis in the house cricket, Acheta domesticus (L.), was examined using an immunodiffusion assay with an estimated sensitivity of 6.3 μg vitellogenin/ml haemolymph. The time course of vitellogenin appearance in adult female haemolymph and analysis of abdominal autonomy for vitellogenin production in females decapitated at various times after the moult revealed that induction does not normally occur during the first 12 h of adult development but is completed by 14–16 h in some females. This is much earlier than expected on the basis of electrophoretic analyses previously reported but correlates with the early rise in juvenile hormone titre recently observed by other workers. Decapitation/hormone replacement experiments using juvenile hormones I and III confirmed the juvenile hormone control of cricket vitellogenin production. Newly moulted adult females were markedly refractory to the inductive effects of juvenile hormone; however, among adult females decapitated at the moult but aged 24 h before injection, 74% produced vitellogenin in response to juvenile hormone. These studies provide the temporal framework for future analyses of juvenile hormone-dependent fat body changes which precede initiation of vitellogenin synthesis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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