Evidence of starch accumulation in tobacco Bright Yellow (TBY-2) cells in the presence of auxin

Autor:	Veronica Ambrosini, Mohammad Issawi, Catherine Riou
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Ecology Starch food and beverages Plant Science Biology Suspension culture chemistry.chemical_compound chemistry Auxin Amylose Amylopectin Botany Cytokinin Ecology Evolution Behavior and Systematics
Zdroj:	Botany. 99:789-794
ISSN:	1916-2804 1916-2790
DOI:	10.1139/cjb-2021-0085
Popis:	Tobacco Bright Yellow (TBY-2) cell suspension is known to produce starch when cultured in a medium supplemented with cytokinin or in a hormone-free medium. Unexpectedly, TBY-2 cells continuously cultivated in the presence of auxin alone were also able to accumulate starch at the beginning of a stationary growth phase, with a yield of 9.22% ± 0.68%. This starch production was strongly correlated with a 25-fold increase in starch synthase activity. Moreover, this TBY-2 line was able to produce an amylopectin-rich starch with a ratio of amylopectin over amylose of 2.7, which was also linked to typical small granules (1.4 µm). According to our preliminary results, this plant cell suspension could yield a low-cost, amylopectin-rich starch — which is needed in the food industry to produce edible film or bioplastic — without being impacted by climate or seasonal weather changes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b1e3f9105633425d92dfddfb488fb819 https://doi.org/10.1139/cjb-2021-0085 Zobrazit plný text záznamu Plný text ve formátu PDF