Enhanced thermotolerance of Arabidopsis by chitooligosaccharides-induced CERK1n-ERc fusion gene
| Autor: | Mengqi Wu, Xiangyang Hu, Jinxing Song, Linxiao Chen, Wei Xia, Wenqing Zhang, Xu Zhizhen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Jasmonic acid Plant Science biology.organism_classification 01 natural sciences Elicitor Cell biology Fusion gene 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Chitin chemistry Germination Arabidopsis Signal transduction Gene 010606 plant biology & botany |
| Zdroj: | Plant Signaling & Behavior. 15:1816322 |
| ISSN: | 1559-2324 |
| Popis: | Heat stress is a major growth-limiting factor for most crops over the world. Chitin elicitor receptor kinase 1 (CERK1) is a chitin/chitooligosaccharides receptor, and ERECTA (ER) plays a crucial role in plant resistance to heat stress. In the present study, a chitooligosaccharides-induced CERK1n-ERc fusion gene was designed and synthesized, in which the extracellular domain and transmembrane domain of CERK1 gene is connected with the response region of ER gene. We successfully constructed the CERK1n-ERc fusion gene by Overlap PCR and introduced it into Arabidopsis by Agrobacterium-medicated infection. Genetically modified (GM) plants had a greater germination rate and germination index, as well as a shorter mean germination time, indicating that they had a better thermotolerance compared with the wild-type (WT) lines under heat stress. Moreover, the GM lines showed a lower level of hydrogen peroxide (H2O2) and relative electrolyte leakage (REL), suggesting that they were in better state compared with the WT plants when exposed to high temperature. UPLC-MS/MS was employed to assess the phytohormone level, suggesting that the GM lines acquired a better thermotolerance via jasmonic acid (JA) signaling pathways. In general, we constructed a COS-induced fusion gene to enhance the thermotolerance of Arabidopsis during seed germination and postgermination growth. |
| Databáze: | OpenAIRE |
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