Utilization of the ORCA to Completely Automate Scintillation Proximity Assays
| Autor: | M. Kelly Talbot, Gordon Powers, Catherine McCaffrey |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
0301 basic medicine
Scintillation business.industry Computer science 01 natural sciences Biochemistry Automation 0104 chemical sciences Analytical Chemistry 010404 medicinal & biomolecular chemistry 03 medical and health sciences 030104 developmental biology Scintillation proximity assay Control data Molecular Medicine Instrumentation (computer programming) business Robotic arm Computer hardware Biotechnology |
| Zdroj: | SLAS Discovery. 1:187-190 |
| ISSN: | 2472-5552 |
| Popis: | A laboratory designed to carry out totally automated high throughput screening (HTS) assays was established recently at the Roche Research Center in Nutley, NJ. The system was designed by Sagian Inc. and uses an optimized robot for chemical analysis (ORCA) robotic arm to transport microplates from station to station. One of the first assays adapted for automation was a scintillation proximity assay (SPA), which screens for inhibitors of binding of a transcription factor to its target DNA. The SPA format was chosen because it is microplate based, and the required instrumentation is available in robot-friendly configurations. In addition to collecting inhibition data, we have compiled control data to assess the quality of results generated by a completely automated HTS system. Generation of usable data began approximately 1 month after the initial setup of the automated system. Results from automated and manual assays of the same compounds correlated well (r = 0.969), confirming the accuracy of the automate... |
| Databáze: | OpenAIRE |
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