Development of an LC/MS/MS method in order to determine arctigenin in rat plasma: its application to a pharmacokinetic study
| Autor: | Guang-Rong Zhao, Changxiao Liu, Quanfei Zou, Yuan Gu, Rong Lu, Tiejun Zhang, Duanyun Si |
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| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
Electrospray Chromatography Chemistry Calibration curve Clinical Biochemistry Selected reaction monitoring Analytical chemistry General Medicine Plasma Biochemistry Analytical Chemistry chemistry.chemical_compound Pharmacokinetics Drug Discovery Lc ms ms Molecular Biology Arctigenin Psoralen |
| Zdroj: | Biomedical Chromatography. 27:1123-1128 |
| ISSN: | 0269-3879 |
| Popis: | In this study, a simple and sensitive LC/MS/MS method was developed and validated for the determination of arctigenin in rat plasma. The MS detection was performed using multiple reaction monitoring at the transitions of m/z 373.2 137.3 for arctigenin and m/z 187.1 131.0 for psoralen (internal standard) with a Turbo IonSpray electrospray in positive mode. The calibration curves fitted a good linear relationship over the concentration range of 0.2–500 ng/mL. It was found that arctigenin is not stable enough at both room temperature and −80 °C unless mixed with methanol before storage. The validated LC/MS/MS method was successfully applied for the pharmacokinetic study of arctigenin in rats. After intravenous injection of 0.3 mg/kg arctigenin injection to rats, the maximum concentration, half-life and area under the concentration–time curve were 323 ± 65.2 ng/mL, 0.830 ± 0.166 and 81.0 ± 22.1 h ng/mL, respectively. Copyright © 2013 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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