LC-MS/MS method development and validation for the determination of antiviral drug in human plasma
| Autor: | Yeluguri Manikanta, S. Jayakumari |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | International journal of health sciences. :7604-7612 |
| ISSN: | 2550-696X 2550-6978 |
| Popis: | The validated protein precipitation method was applied to estimate Darunavir in human plasma with Verapamil as an internal standard. The analytical column was selected Agilent, Zorbax, and XDB C18 (2.1 x 50 mm ID, 5 μm). The column temperature was set at 30°C. The mobile phase composition was acetonitrile:2Mm Ammonium Formate with 0.1% formic acid in water (70:30 v/v). Source flow rate at 0.120 mL/min without a split. An injection volume of 10 µL. Darunavir and Verapamil were eluted at 1.35 &1.13 min, with a total run time of 3.0 min for each sample |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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