Comparative evaluation of different diagnostic tests for B. gibsoni in dogs
| Autor: | Karam Pal Singh, Rashmi Ranjan Mahapatra, D. B. Mondal, Neelam Kushwaha |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Diagnostic methods General Veterinary Babesia gibsoni business.industry 030231 tropical medicine Canine babesiosis Diagnostic test Babesiosis Parasitemia 030108 mycology & parasitology medicine.disease Comparative evaluation 03 medical and health sciences 0302 clinical medicine Blood smear parasitic diseases medicine Animal Science and Zoology business |
| Zdroj: | Indian Journal of Animal Research. |
| ISSN: | 0976-0555 0367-6722 |
| DOI: | 10.18805/ijar.b-3413 |
| Popis: | Babesia gibsoni is the common cause of canine babesiosis in India. Its diagnosis by conventional diagnostic methods result in false negative condition, particularly in those cases in which parasitemia is low. Therefore the present study employed IFAT and PCR along with microscopic examination to overcome this situation. Microscopic examination of Giemsa-stained blood smears revealed presence of small pear-shaped, oval or signet ring shaped B. gibsoni within the erythrocytes of 1.34 % dogs. IFAT and PCR detected a total of 1.80 % and 1.82 % dogs positive for B. gibsoni, respectively. The sensitivity and specificity of IFAT was 93.10% and 88.89%, respectively whereas, sensitivity and specificity of microscopic examination was 71.26% and 95.56%, respectively. The agreement of IFAT and microscopic examination with PCR was 82.30% and 60.73%, respectively. The agreement between IFAT and microscopy was 60.99%. Therefore, IFAT is considered to be highly sensitive test for diagnosing B. gibsoni in subclinically or chronically infected dogs with significantly low level of parasitemia. |
| Databáze: | OpenAIRE |
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