Aortic Sca-1+Progenitor Cells Arise from the Somitic Mesoderm Lineage in Mice
| Autor: | Angela Li, Sarah K. Steinbach, Martha H Carruthers, Clinton S. Robbins, Mansoor Husain, Adam P. Johnston, Rickvinder Besla, Tao Wang |
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| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Stem Cells and Development. 27:888-897 |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2018.0038 |
| Popis: | Sca-1+ progenitor cells in the adult mouse aorta are known to generate vascular smooth muscle cells (VSMCs), but their embryological origins and temporal abundance are not known. Using tamoxifen-inducible Myf5-CreER mice, we demonstrate that Sca-1+ adult aortic cells arise from the somitic mesoderm beginning at E8.5 and continue throughout somitogenesis. Myf5 lineage-derived Sca-1+ cells greatly expand in situ, starting at 4 weeks of age, and become a major source of aortic Sca-1+ cells by 6 weeks of age. Myf5-derived adult aortic cells are capable of forming multicellular sphere-like structures in vitro and express the pluripotency marker Sox2. Exposure to transforming growth factor-β3 induces these spheres to differentiate into calponin-expressing VSMCs. Pulse-chase experiments using tamoxifen-inducible Sox2-CreERT2 mice at 8 weeks of age demonstrate that ∼35% of all adult aortic Sca-1+ cells are derived from Sox2+ cells. The present study demonstrates that aortic Sca-1+ progenitor cells are derived from the somitic mesoderm formed at the earliest stages of somitogenesis and from Sox2-expressing progenitors in adult mice. |
| Databáze: | OpenAIRE |
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