A Novel MspI PCR-RFLP in the Human Cytosine 5-Methyltransferase Gene: Lack of Relevance for Malignant Lymphoproliferative Disease and Breast Cancer

Autor:	Mohammed O. Ahmed, Aparna N. Bagwe, John Papadimitriou, Peter Kay, M. Franchina, Dominic V. Spagnolo
Rok vydání:	1998
Předmět:	Genetics Biology Molecular biology Gene dosage law.invention genomic DNA chemistry.chemical_compound chemistry law Copy-number variation Restriction fragment length polymorphism Gene Genotyping Genetics (clinical) Polymerase chain reaction Cytosine
Zdroj:	Human Heredity. 48:226-229
ISSN:	1423-0062 0001-5652
DOI:	10.1159/000022805
Popis:	Two alternate allelic forms of human cytosine 5-methyltransferase, 5-MT I and 5-MT II, which differ by the absence or presence of an intronic MspI recognition sequence, have been recognised. The polymorphic region was localised using a series of subprobes prepared upon MspI digestion of the 2.5-kb cDNA probe (hmt-2.5). A PCR-based method was then developed for rapid 5-MT genotyping. The gene and phenotype frequencies of 5-MT I and 5-MT II were not significantly different in genomic DNA samples from a series of non-Hodgkin’s lymphomas and breast cancer cases compared with DNA from normal subjects. Allelism of 5-MT allows new approaches to the assessment of variation in gene copy number of 5-MT in different types of neoplasia.
Databáze:	OpenAIRE
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