Characterization and cytocompatibility of a new injectable multiphasic bone substitute based on a combination of polysaccharide gel-coated OSPROLIFE®HA/TTCP granules and bone marrow concentrate
| Autor: | Eleonora Preve, Michela Pierini, Susanna Prosperi, Marzio Piccinini, Francesco Bucciotti, Enrico Lucarelli, Davide Maria Donati, Serena Duchi |
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| Rok vydání: | 2015 |
| Předmět: |
Materials science
0206 medical engineering Mesenchymal stem cell Biomedical Engineering ALIZARIN RED Tetracalcium phosphate ttcp 02 engineering and technology Matrix (biology) 021001 nanoscience & nanotechnology Bone tissue 020601 biomedical engineering Staining Biomaterials chemistry.chemical_compound medicine.anatomical_structure chemistry medicine Bone marrow 0210 nano-technology Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. 104:894-902 |
| ISSN: | 1552-4973 |
| DOI: | 10.1002/jbm.b.33441 |
| Popis: | The purpose of this study was to examine the in vitro cytocompatibility of a novel injectable multiphasic bone substitute (MBS) based on polysaccharide gel-coated OSPROLIFE(®) hydroxyapatite (HA)/tetracalcium phosphate (TTCP) granules combined with bone marrow concentrate (BMC). Polysaccharide gel-coated granules loaded in syringe were combined with BMC diluted in ionic crosslinking solution. The product was then maintained in culture to investigate the cytocompatibility, distribution, and osteogenic differentiation function of cells contained in the BMC. The in vitro cytocompatibility was assessed after 0, 24, and 96 h from the injectable MBS preparation using the LIVE/DEAD(®) staining kit. The results highlighted that cells remained viable after combination with the polysaccharide gel-coated granules; also, viability was maintained over time. The distribution of the cells in the product, observed using confocal microscopy, showed viable cells immersed in the polysaccharide gel formed between the granules after ionic crosslinking. The mesenchymal stromal cells (MSC) contained in the injectable MBS, the basic elements for bone tissue regeneration, were able to differentiate toward osteoblasts, producing an osteogenic matrix as evidenced by alizarin red-s (AR-S) staining. In conclusion, we found that the injectable MBS may have the potential to be used as a bone substitute by applying a "one-step" procedure in bone tissue engineering applications. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 894-902, 2016. |
| Databáze: | OpenAIRE |
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