catAGene in a PotentialCorynebacteriumStrain is Responsible for its Efficiency in Phenol Bioremoval

Autor:	D. P. Panigrahi, G. S. Randhawa, S. Dalal, R. C. Dubey
Rok vydání:	2012
Předmět:	Catechol Chromatography Polymers and Plastics biology Organic Chemistry Corynebacterium biology.organism_classification chemistry.chemical_compound Bioremediation chemistry Dioxygenase visual_art Materials Chemistry visual_art.visual_art_medium Phenol Organic chemistry Sawdust Effluent Bacteria
Zdroj:	Polycyclic Aromatic Compounds. 32:423-438
ISSN:	1563-5333 1040-6638
Popis:	Out of 10 bacterial strains isolated out of wastewater of a pharmaceutical industry the most efficient bacterium, which could tolerate 5,000 mgl−1 of phenol in the minimal medium, was identified to be Corynebacterium sp.DST1 through 16S rDNA analysis. This strain could remove 99.4% of phenol from the minimal medium containing 4,000 mgl−1 of phenol. The ortho catalytic pathway using the enzyme catechol 1, 2- dioxygenase was found to be the major pathway for phenol degradation in the bacterium. The gene for catechol 1, 2-dioxygenase (catA) could also be amplified using degenerate primers. Corynebacterium sp.DST1 cells were attached to sawdust and removal of phenol from industrial effluents was evaluated. About 92.7% of phenol could be removed from the effluent of a heavy electrical industry. The results of this study not only suggest the potentiality of the strain to remove phenol efficiently from crude industrial effluents but also display a cheap method of bioremediation using sawdust.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b1b8dfa29d83d5c31a16e78b116bbb77 https://doi.org/10.1080/10406638.2011.645267 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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