A fast and reliable chromatographic procedure for the purification of virginiamycin M1 factor
| Autor: | Francis Gossele, Michel Paquot, Katherine Nott, Sylvie Heilporn, Georges Lognay, Jean Giard, Bernard Wathelet, Pascal Gerbaux |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Chromatography
Chemistry Silica gel Organic Chemistry Clinical Biochemistry Infrared spectroscopy Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry law.invention chemistry.chemical_compound Column chromatography law medicine Virginiamycin Filtration Antibacterial agent medicine.drug |
| Zdroj: | Chromatographia. 56:331-335 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1007/bf02491941 |
| Popis: | The goal of this study was to develop a simple, fast and reliable procedure for the preparation of highly purified virginiamycin M1 factor using readily available and low cost laboratory equipment. This was achieved by purifying M1 by reversed-phase flash chromatography after clean-up of the bulk sample by filtration on a silica gel cake. The purity of the final product determined by RP-HPLC was 99% and its identity was confirmed by IR spectroscopy and ESI mass spectrometry. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink