Isolation, Culture, Functional Assays, and Immunofluorescence of Myofiber-Associated Satellite Cells
| Autor: | Adam B. Cadwallader, Tiffany Elston, Thomas O. Vogler, Katherine E. Gadek, Bradley B. Olwin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
biology Cellular differentiation Skeletal muscle biology.organism_classification Cell biology Extensor digitorum longus muscle Transplantation 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Cell culture medicine Myocyte Satellite (biology) Stem cell |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493938087 |
| DOI: | 10.1007/978-1-4939-3810-0_11 |
| Popis: | Adult skeletal muscle stem cells, termed satellite cells, regenerate and repair the functional contractile cells in adult skeletal muscle called myofibers. Satellite cells reside in a niche between the basal lamina and sarcolemma of myofibers. Isolating single myofibers and their associated satellite cells provides a culture system that partially mimics the in vivo environment. We describe methods for isolating and culturing intact individual myofibers and their associated satellite cells from the mouse extensor digitorum longus muscle. Following dissection and isolation of individual myofibers we provide protocols for myofiber transplantation, satellite cell transfection, immune detection of satellite cell antigens, and assays to examine satellite cell self-renewal and proliferation. |
| Databáze: | OpenAIRE |
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