The inhibitory effect of xylan on enzymatic hydrolysis of cellulose is dependent on cellulose ultrastructure
Autor: | Hailong Li, Xinde Chen, Can Wang, Xindong Chen, Ge Yuan, Liquan Zhang, Chen Xuefang, Lian Xiong |
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Rok vydání: | 2020 |
Předmět: |
Polymers and Plastics
biology Ethylenediamine 02 engineering and technology Cellobiose Cellulase 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Xylan 0104 chemical sciences chemistry.chemical_compound Hydrolysis chemistry Enzymatic hydrolysis biology.protein Bioorganic chemistry Cellulose 0210 nano-technology Nuclear chemistry |
Zdroj: | Cellulose. 27:4417-4428 |
ISSN: | 1572-882X 0969-0239 |
Popis: | The inhibitory effect of xylan on enzymatic hydrolysis of cellulose with different ultrastructure is not clear. In this work, cellulose II, cellulose III, and amorphous cellulose were prepared from cellulose I by NaOH, ethylenediamine, and ball-milling treatment, respectively. Their crystalline structure and enzymatic hydrolyzability were systematically characterized and compared with cellulose I. Furthermore, the inhibitory effect of xylan on cellulose with different ultrastructure was investigated in detail. Results suggested that the enzymatic hydrolyzability of different cellulose increased in sequence from cellulose I (25.4%), cellulose III (41.7%), cellulose II (56.2%) to amorphous cellulose (77.4%) at enzyme loadings of 5.56 mg protein/g substrate. The enzymatic hydrolyzability of allomorphic cellulose was strongly inhibited by xylan, while a little inhibition was observed for amorphous cellulose. It was found that cellobiose released from different cellulose was decreased after the addition of xylan. At the initial hydrolysis stage, the inhibition degree was obviously increased for all of different cellulose, and then, the inhibition degree of cellulose I and cellulose II gradually decreased as enzymatic hydrolysis proceeded, while the inhibition degree of cellulose III and amorphous cellulose had changed a little. In addition, at a longer time of hydrolysis, the inhibition degree was decreased with the increase of cellulase dosage except for cellulose III, which remained approximately unchanged. Overall, this study demonstrated that the inhibitory effect of xylan on cellulose hydrolysis was substantially affected by the ultrastructure of cellulose, and thus it provides new insights for relieving the inhibitory effect of xylan by altering the ultrastructure of cellulose. |
Databáze: | OpenAIRE |
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