Metal affinity extraction of human hemoglobin in an aqueous polyethylene glycol-sodium sulfate two-phase system
| Autor: | S. D. Plunkett, F. H. Arnold |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Aqueous solution
Chromatography biology technology industry and agriculture Serum albumin Polyethylene glycol Human serum albumin Applied Microbiology and Biotechnology Biochemistry Metal chemistry.chemical_compound chemistry visual_art PEG ratio Sodium sulfate visual_art.visual_art_medium medicine biology.protein Hemoglobin medicine.drug |
| Zdroj: | Biotechnology Techniques. 4:45-48 |
| ISSN: | 1573-6784 0951-208X |
| Popis: | We have developed a protein extraction technique which uses metal affinity ligands in PEG/salt aqueous two-phase systems. Cu(II)IDA-PEG will partition proteins according to their surface histidine contents in two-phase systems formed from sodium sulfate and polyethylene glycol. The nearly complete separation of human hemoglobin and human serum albumin in a single stage is presented as a demonstration of the effectiveness of metal affinity extraction in PEG/salt systems. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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