Resolution of amino acid enantiomers by high-performance liquid chromatography using automated pre-column derivatisation with a chiral reagent

Autor:	R.H. Buck, K. Krummen
Rok vydání:	1984
Předmět:	chemistry.chemical_classification Chromatography Resolution (mass spectrometry) Chemistry Organic Chemistry Diastereomer General Medicine Biochemistry High-performance liquid chromatography Fluorescence Analytical Chemistry Amino acid Reagent Enantiomer Cysteine
Zdroj:	Journal of Chromatography A. 315:279-285
ISSN:	0021-9673
DOI:	10.1016/s0021-9673(01)90745-9
Popis:	A new and highly sensitive method for the determination of amino acid enantiomers is presented. Fluorescent diastereoisomers are formed by reaction with o -phthaldialdehyde and Boc- l -cysteine prior to chromatography. The reaction occurs rapidly at ambient temperatures and is performed by an automated pre-column derivatisation device integrated into the high-performance liquid chromatographic system. Separation of the diastereoisomers is carried out by reversed-phase chromatography with gradient elution and the derivatives are detected fluorimetrically. Complete resolutions were observed for the enantiomers of all 21 amino acids examined. The method allows the determination of individual amino acids, including the enantiomeric forms, in a single chromatographic run.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b17efc367990aa4274ea7495ec1def2a https://doi.org/10.1016/s0021-9673(01)90745-9 Zobrazit plný text záznamu