Induction of autotetraploidy using colchicine and its identification in radish (Raphanus sativusL.)
| Autor: | Y. Sha, Cecilia Limera, Yuhua Wang, Y. Gong, L. Liu, Lijuan Xu, Kaituo Wang, H. Feng, X. Zhu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology fungi food and beverages Raphanus Chromosome Horticulture Meristem biology.organism_classification 01 natural sciences Chloroplast 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Inbred strain chemistry Guard cell Botany Genetics Colchicine 010606 plant biology & botany Stomatal density |
| Zdroj: | The Journal of Horticultural Science and Biotechnology. 91:63-70 |
| ISSN: | 2380-4084 1462-0316 |
| DOI: | 10.1080/14620316.2015.1110993 |
| Popis: | Radish (Raphanus sativus L.) is an important root vegetable in the family Brassicaceae, and is popular worldwide, especially in East Asia. Colchicine was used to induce autotetraploidy in four advanced inbred lines of radish. A combination of 0.1% (w/v) colchicine in 0, 0.1%, 0.2%, or 0.4% (v/v) dimethyl sulphoxide (DMSO) was applied to the apical meristem of young seedlings. A preliminary screening for putative tetraploids was conducted based on morphological traits including flower size, the number of chloroplasts in guard cells, stomatal size, and stomatal density. Plants with significantly larger stomata, with more chloroplasts in their guard cells, were selected for further analysis by chromosome counting. Only one tetraploid genotype, ‘Nau-dy13’, was successfully induced using 0.1% (w/v) colchicine plus 0.2% (v/v) DMSO, with a tetraploid induction rate of 20%. Application of the above concentrations of colchicine and DMSO to the apical meristem of ‘Nau-dy13’ seedlings was found to be an effi... |
| Databáze: | OpenAIRE |
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