Development of a biosafety level-2 facility for irradiation of biological cells using MeV ions
| Autor: | Harry J. Whitlow, Naresh T. Deoli, Robin Schneider-Broussard, Deborah Rogers, Karen Müller Smith, Armin B. de Vera, Jay D. Dias |
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| Rok vydání: | 2019 |
| Předmět: |
Nuclear and High Energy Physics
Materials science 010504 meteorology & atmospheric sciences Radiochemistry 01 natural sciences Fluence Ion Biosafety Low energy Biosafety level 0103 physical sciences Biological cell Irradiation 010303 astronomy & astrophysics Instrumentation 0105 earth and related environmental sciences |
| Zdroj: | Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. 447:50-54 |
| ISSN: | 0168-583X |
| DOI: | 10.1016/j.nimb.2019.03.002 |
| Popis: | The development of a facility for irradiation of murine and primate tissue presents several challenges. First, extremely low fluences (106–1011 ions cm−2) are required to deliver doses of 1 mGy–50 Gy using MeV proton. Second, the tissues, particularly from non-human primate, may be infectious to humans. Third, protection of irradiated tissue samples from contamination by mold and bacteria is critical. Here we present an ultra-low flux irradiation facility and associated fluence control for biological cell and tissue irradiation under sterile biosafety level-2 conditions suitable for use with low energy (1–3.4 MeV) protons. The operation is demonstrated using murine astrocytes. |
| Databáze: | OpenAIRE |
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