SCREENING OF BIOPROTECTIVE PROPERTIES OF VARIOUS PLANT EXTRACTS AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY PROFILING OF ADENANTHERA PAVONINA STEM EXTRACT
| Autor: | Shubha Bhadran, Sangeetha Annie George, Sudhakar Malla, Harini Bp |
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| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Asian Journal of Pharmaceutical and Clinical Research. 10:188 |
| ISSN: | 2455-3891 0974-2441 |
| DOI: | 10.22159/ajpcr.2017.v10i7.18141 |
| Popis: | Objective: The search for various phytotherapeutic compounds is on rise due to a complex multifactorial phenomenon called drug resistance. The present study investigates the cytotoxic, antioxidant, and antiproliferative potential of methanolic extracts of Clitorea ternatea, Averrhoa bilimbi, Phyllanthus acidus, Tecoma stans, Curcuma aromatica, Anethum graveolens, Adhatoda vasica, Markhamia lutea, Spathodea companulata, and Adenanthera pavonina.Methods: The plant parts were extracted with methanol and screened for 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging abilities. The cytotoxic activity of the extracts was investigated on HeLa and HCT116 cells through 3-(4,5-dimethylthiazol2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, and cell cycle was analyzed by flow cytometry to determine the antiproliferative activity of the extracts. The stem extract of A. pavonina was further subjected to gas chromatography-mass spectrometry (GC-MS) analysis for purification of the compounds of interest. A two-way ANOVA was done to estimate the effect of the extract between samples remembered at p |
| Databáze: | OpenAIRE |
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