Isolation, characterization and cross-species amplification of new microsatellite markers for three opossum species of the Didelphidae family

Autor:	Jennifer K. Peterson, Isabela M. G. Dias, Heitor M. Cunha, Rob DeSalle, Adriano P. Paglia, George Amato, C. G. Fonseca
Rok vydání:	2009
Předmět:	Genetics education.field_of_study biology Population Caluromys philander Zoology biology.organism_classification Caluromyinae Metachirus nudicaudatus Micoureus paraguayanus IUCN Red List Microsatellite Didelphis aurita education Ecology Evolution Behavior and Systematics
Zdroj:	Conservation Genetics Resources. 1:405-410
ISSN:	1877-7260 1877-7252
DOI:	10.1007/s12686-009-9094-8
Popis:	The American opossums from the Didelphidae family (Order: Didelphimorphia) are on the IUCN Red List, and their threatened status varies from extinct to data deficient. Although microsatellites have already been developed for some species, only a small amount of population genetic analysis of the didelphid wild populations has been made using them. In this work, we developed new microsatellites and tested for cross-species amplification for one species from the subfamily Caluromyinae, Caluromys philander, and two from the subfamily Didelphinae, Didelphis aurita and Metachirus nudicaudatus, to determine the degree of gene flow and isolation of populations of those species in some fragments of the Atlantic Forest in Minas Gerais state, Brazil. Among all microsatellite loci amplified, 18 from C. philander, 9 from D. aurita, and 17 from M. nudicaudatus were polymorphic. Cross-species amplification of these polymorphic microsatellite loci were performed in these three species and in one additional related species Micoureus paraguayanus. Our data showed that microsatellites were not highly conserved among Didelphidae species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b1429c614130311bba1dd255efffae10 https://doi.org/10.1007/s12686-009-9094-8 Zobrazit plný text záznamu