Monitoring cysteine level changes under LPS or H2O2 induced oxidative stress using a polymer-based ratiometric fluorescent probe
| Autor: | Weiying Lin, Faqi Yu, Xinying Jing |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Redox homeostasis biology 010401 analytical chemistry 02 engineering and technology Polymer 021001 nanoscience & nanotechnology medicine.disease_cause biology.organism_classification 01 natural sciences Biochemistry Fluorescence 0104 chemical sciences Analytical Chemistry Amino acid chemistry medicine Biophysics Environmental Chemistry 0210 nano-technology Zebrafish Spectroscopy Oxidative stress Intracellular Cysteine |
| Zdroj: | Analytica Chimica Acta. 1174:338738 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2021.338738 |
| Popis: | Cysteine (Cys) is a critical amino acid that involves in many physiological and pathological processes in the human body, and it plays an important role in maintaining redox homeostasis in living systems. The concentration of intracellular Cys is abnormal under oxidative stress thus leading to many diseases. Therefore, it is significant to develop an effective method for detection of Cys under oxidative stress. In this work, we propose a new polymer-based ratiometric fluorescent probe with good selectivity and sensitivity for detecting Cys. The bioimaging experiments results show that the novel probe has a rapid ratiometric response to Cys, which can be used to monitor Cys level changes during LPS or H2O2 induced oxidative stress in living cells and zebrafish. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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