Determination of cyclohexamone after derivatization with 2,4-dinitrophenyl hydrazine in intravenous solutions stored in PVC bags by high performance liquid chromatography
| Autor: | Thierry Dine, L. Ballester, B. Gressier, Jean-Claude Cazin, Micheline Cazin, F. Khalfi, C. Brunet, M. Luyckx |
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| Rok vydání: | 1998 |
| Předmět: |
Pharmacology
Detection limit Aqueous solution Chromatography Clinical Biochemistry Hydrazine Cyclohexanone General Medicine Biochemistry High-performance liquid chromatography Vinyl chloride Analytical Chemistry Pentane chemistry.chemical_compound chemistry Drug Discovery Derivatization Molecular Biology |
| Zdroj: | Biomedical Chromatography. 12:69-72 |
| ISSN: | 1099-0801 0269-3879 |
| Popis: | A high performance liquid chromatographic procedure is described for the determination of cyclohexanone leached in intravenous solutions from the poly(vinyl chloride) bags. After derivatization with 2,4-dinitrophenylhydrazine and extraction with pentane, the cyclohexanone derivative was analysed on a C18 BDS Hypersil column using mobile phase mixture of acetonitrile:water (55:45). Ultra-violet detection was performed at 368 nm. The limit of quantification was 30 ng/mL and the assay was linear from 0.05 to 50 micrograms/mL. The recovery was better than 95%. The proposed method is satisfactory in its accuracy and precision with particularly relative standard deviations (RSD) for intra-assay and inter-assay of below 10%. This method has been successfully used for the determination of cyclohexanone in aqueous solutions such as sodium chloride (0.9%) and glucose (5%) stored in PVC containers. The values obtained varied between 2.04 and 44.9 micrograms/mL according to solutions and volume. |
| Databáze: | OpenAIRE |
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