| Autor: |
Vasanth S. Murali, Erik S. Welf, Murat Can Cobanoglu |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Methods in Molecular Biology ISBN: 9781071612040 |
| DOI: |
10.1007/978-1-0716-1205-7_12 |
| Popis: |
Researchers often aim to incorporate microenvironmental variables such as the dimensionality and composition of the extracellular matrix into their cell-based assays. A technical challenge created by introduction of these variables is quantification of single-cell measurements and control of environmental reproducibility. Here, we detail a methodology to quantify viability and proliferation of melanoma cells in 3D collagen-based culture platforms by automated microscopy and 3D image analysis to yield robust, high-throughput results of single-cell responses to drug treatment. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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