Studies on the mechanism of multidrug resistance of Acinetobacter baumannii by proteomic analysis of the outer membrane vesicles of the bacterium
| Autor: | Bina Agarwal, Giasuddin Ahmed, Medicharla V. Jagannadham, P Gayathri, B. RameshBabu, Raman Karthikeyan |
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| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
biology 030306 microbiology medicine.drug_class Chemistry Antibiotics biology.organism_classification Microbiology Acinetobacter baumannii Multiple drug resistance 03 medical and health sciences Minimum inhibitory concentration Antibiotic resistance medicine Bacterial outer membrane Bacteria Polymyxin B 030304 developmental biology medicine.drug |
| Zdroj: | Journal of Proteins and Proteomics. 10:1-15 |
| ISSN: | 2524-4663 0975-8151 |
| DOI: | 10.1007/s42485-018-0001-4 |
| Popis: | Acinetobacter baumannii, an opportunistic pathogen is responsible for pneumonia, meningitis and a wide range of other infections. It is typically involved in nosocomial infections. Outer membrane vesicles (OMVs) released from the bacteria play an important role in the bacterial physiology including pathogenesis. In this study, OMVs were isolated and characterized form sensitive and multidrug-resistant clinical strains of A. baumannii. A comparative proteomics analysis of the OMVs from the A. baumannii sensitive and MDR strain revealed the increased content of carbapenemase and a host of other related antibiotic resistant enzymes in the OMVs of the A. baumannii MDR strain. The OMVs of antibiotic-sensitive strain contained only 8 antibiotic resistance-conferring proteins, whereas the OMVs of multidrug resistance contained 24 proteins. Growth studies on the sensitive and MDR strain in the presence of polymyxin B and OMVs prepared from the respective strains revealed that the OMVs protected the bacteria against the antibiotic. Bacteria were grown in the presence of antibiotic and an indole derivative has shown that the minimum inhibitory concentration was reduced in the presence of derivative indicating that the derivative regulated the production of OMVs. Interestingly, it was also found that the degradosome complex involved in RNA processing was associated with the OMVs. These studies reveal that OMVs may play an important role in antibiotic resistance/persistence. |
| Databáze: | OpenAIRE |
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