Diapause-associated changes in the lipid and metabolite profile of the Asian tiger mosquito, Aedes albopictus
| Autor: | Peter Armbruster, Zachary A. Batz |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Aedes albopictus biology Physiology Metabolite Zoology Aquatic Science Diapause biology.organism_classification 010603 evolutionary biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Insect Science Lipid droplet Lipidomics Tiger mosquito Animal Science and Zoology Embryonic diapause Adaptation Molecular Biology Ecology Evolution Behavior and Systematics |
| Zdroj: | Journal of Experimental Biology. |
| ISSN: | 1477-9145 0022-0949 |
| DOI: | 10.1242/jeb.189480 |
| Popis: | Diapause is an alternative life-history strategy that allows organisms to enter developmental arrest in anticipation of unfavorable conditions. Diapause is widespread among insects and plays a key role in enhancing overwinter survival as well as defining the seasonal and geographic distributions of populations. Next generation sequencing has greatly advanced our understanding of the transcriptional basis for this crucial adaptation but less is known about regulation of embryonic diapause physiology at the metabolite level. Here, we characterized the lipid and metabolite profile of embryonic diapause in the Asian tiger mosquito, Aedes albopictus. We used an untargeted approach to capture the relative abundance of 250 lipids and 241 metabolites. We observed adjustments associated with increased energy storage, including an accumulation of lipids, the formation of larger lipid droplets, and increased lipogenesis, as well as metabolite shifts suggesting reduced energy utilization. We also found changes in neuroregulatory- and insulin-associated metabolites with potential roles in diapause regulation. Finally, we detected a group of unidentified, diapause-specific metabolites which have similar physical properties steroids/steroid derivatives and may be associated with the ecdysteroidal regulation of embryonic diapause in Ae. albopictus. Together, these results deepen our understanding of the metabolic regulation of embryonic diapause and identify key targets for future investigations. |
| Databáze: | OpenAIRE |
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