Sustained neuroprotection after a single intravitreal injection of PGJ2 in a rodent model of NAION
Autor: | Yan Guo, Sl Bernstein, N. R. Miller, Mary A. Johnson, V. Touitou |
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Rok vydání: | 2010 |
Předmět: |
Retina
medicine.medical_specialty genetic structures business.industry Retinal General Medicine medicine.disease Neuroprotection Surgery Ophthalmology chemistry.chemical_compound medicine.anatomical_structure Retinal ganglion cell chemistry Toxicity medicine Optic nerve Anterior ischemic optic neuropathy business Stroke |
Zdroj: | Acta Ophthalmologica. 88 |
ISSN: | 1755-375X |
DOI: | 10.1111/j.1755-3768.2010.2121.x |
Popis: | Purpose Prostaglandin-J2 (PGJ2) has been proposed as a potential neuroprotective agent. We wanted to evaluate the toxicity/efficacy of a single intravitreal (IVT) injection of PGJ2 in a rodent model of nonarteritic anterior ischemic optic neuropathy (NAION). Methods We used the laser-activated rose Bengal induction method to produce AION in Long-Evans rats. We evaluated IVT-PGJ2 retinal and ON toxicity. Following induction, PGJ2 was IVT-injected in the treatment-group. IVT phosphate-buffered-saline (PBS) was used as control. Functional studies (VEP) were performed at baseline and at 7days post-treatment. Structural studies included immunohistochemical (IHC), electron microscopic (EM) analysis of the optic nerve (ON), and stereologic analysis of retinal ganglion cell (RGC) numbers at30 day 30. Results Toxicity: IVT PGJ2 (5 eyes) did not induce any significant functional/structural changes in the retina or ON of treated animals compared with animals injected with PBS (5 eyes) 30 days post-injection. Efficacy: After a single IVT-injection, day7 VEPs in the PGJ2-treatment group (n=7) had amplitudes 103.6% of baseline, whereas the PBS-treated group (n=6) had VEPs that were 42.4% of the baseline. 30days post-stroke, EM of ON from the treatment-group demonstrated significant preservation of axons and decreased demyelination. Stereological RGCcounts confirmed significant (p |
Databáze: | OpenAIRE |
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