Clinical effectiveness of the Du Pont aca measurement of creatine kinase MB in serum from patients in a coronary-care unit
| Autor: | Paul R. E. Desjardins, Michael Leroux, John Rabson |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
medicine.medical_specialty
Chromatography Receiver operating characteristic biology Clinical effectiveness business.industry Biochemistry (medical) Clinical Biochemistry Urology medicine.disease Creatine kinase.MB chemistry.chemical_compound chemistry Lactate dehydrogenase medicine biology.protein Coronary care unit Creatine kinase Myocardial infarction business Coronary intensive care |
| Zdroj: | Clinical Chemistry. 30:1552-1554 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/30.9.1552 |
| Popis: | We evaluated the clinical effectiveness of measuring creatine kinase (CK; EC 2.7.3.2) isoenzyme MB and lactate dehydrogenase (LD; EC 1.1.1.27) isoenzymes in diagnosis of acute myocardial infarction. We used an agarose electrophoresis method to measure CK and LD isoenzymes and the Du Pont aca column method to measure CK-MB. Serial blood specimens were drawn from 100 patients consecutively admitted to our Coronary Care Unit. Because of the low diagnostic specificity for CK-MB measurements by both agarose electrophoresis and the discrete-analysis method, as compared with reported values, we re-evaluated our isoenzyme data by using Receiver Operating Characteristic curves. Such analysis of the data established optimal decision levels of greater than or equal to 25 U/L and greater than or equal to 18 U/L plus greater than or equal to 6% of total CK for serum CK-MB measured by the agarose electrophoresis and the aca methods, respectively, and an optimal decision level of greater than or equal to 0.92 for the ratio of LD 1/2 measured after agarose electrophoresis. At these decision levels we obtained a sensitivity of 100%, 100%, and 95% and a specificity of 94%, 92%, and 90% for CK-MB (agarose electrophoresis), CK-MB (aca), and the LD 1/2 ratio, respectively. |
| Databáze: | OpenAIRE |
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