Performing DNA computation with RecA-mediated triple-stranded DNA structure

Autor:	Gang Fang, Jin Xu, Shemin Zhang
Rok vydání:	2007
Předmět:	Genetics Streptavidin Computation RecA Protein Triple-stranded DNA Computer Science Applications law.invention chemistry.chemical_compound chemistry DNA computing law Biotinylation Homologous chromosome Biophysics DNA Mathematics
Zdroj:	Natural Computing. 8:149-156
ISSN:	1572-9796 1567-7818
Popis:	A new method of DNA computing using an unusual triple-stranded DNA structure mediated by RecA protein is introduced and a minor 3-variable instance of the satisfiability (SAT) problem is solved. Separations were performed by using biotinylated oligodeoxynucleotide probes coated with RecA protein. During the computation, sequence-specific recognition of double-stranded DNA by homologous oligodeoxynucleotides was fulfilled in the presence of ATPγS. Captured by the magnetic particles coated with streptavidin, the desirable double-stranded DNA strands were retained and extracted. It is suggested that the methods employed here may help decrease the errors in DNA computation and solve some larger size NP- complete problems.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b0ef41e5b106df2fdd1d92e87783fdb0 https://doi.org/10.1007/s11047-007-9060-x Zobrazit plný text záznamu Full text from SpringerLink