| Autor: |
Adinarayana Kunamneni, Poluri Ellaiah, Thaer Taleb Abed Abdelghani |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
American Journal of Immunology. 1:125-129 |
| ISSN: |
1553-619X |
| DOI: |
10.3844/ajisp.2005.125.129 |
| Popis: |
Of 103 β-haemolytic Streptococci (with haemolytic activity) in total were isolated from different samples of blood and biomass from infected throat, 47 isolates exhibited streptokinase (SK) activity. T3, identified as a new variant of Streptococcus equisimilis, was found to be excellent SK producer in submerged fermentation and was selected for strain improvement studies. This strain was mutagenized by UV and N-methyl-N'-nitro-N-nitroso guanidine (NTG). The isolation of mutants and the SK activity of selected mutants were described. The SK yield of the best UV mutant AUV10 was 120% higher than the wild strain (T3). Also, the SK yield of the best NTG mutant NUV7 was 146% higher than the wild strain (T3). The results indicated that UV and NTG were effective mutagenic agents for strain improvement of S. equisimilis T3 for enhanced SK productivity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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