Cellulase production induced by carbon sources derived from waste newspaper

Autor: Shu Chen, Morris Wayman
Rok vydání: 1991
Předmět:
Zdroj: Process Biochemistry. 26:93-100
ISSN: 1359-5113
DOI: 10.1016/0032-9592(91)80023-i
Popis: Inducers derived from waste newspaper (or discarded office paper) were investigated for their use in cellulase production by T. reesei . Partially enzymatically saccharified newspaper was found to be most effective. When 1·0 g newspaper and 0·07 g sorbose were used for cellulase productions, the cellulases so produced after 6 days of fermentation saccharified 8·0 g waste white office paper in 9·34% suspension, resulting in 5·4 g fermentable sugars, or 6·3 g fermentable sugars with supplementation of β-glucosidase. Additional cellulases were produced from the cells and residual newspaper separated from the sixth day fermentation broth, and were effective in saccharifying another 8·0 g office paper. A total yield of 10–11 g fermentable sugars was obtained, from a total of 16 g office paper, being saccharified by the cellulase induced by 1 g partially saccharified newspaper. Effects of metabolizability of the inducers and addition of sorbose on cellulase production, kinetic behaviour of cellulase production, and comparison with commercial cellulases are presented.
Databáze: OpenAIRE