Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9
| Autor: | Xiaonan Ma, Xiaoyan Zhang, Huimin Liu, Zhenghe Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine food and beverages Mutagenesis (molecular biology technique) RNA RNA virus Plant Science Computational biology Biology biology.organism_classification 01 natural sciences Genome Viral vector 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Genome editing CRISPR DNA 010606 plant biology & botany |
| Zdroj: | Nature Plants. 6:773-779 |
| ISSN: | 2055-0278 |
| Popis: | Genome-editing technologies using CRISPR–Cas nucleases have revolutionized plant science and hold enormous promise in crop improvement. Conventional transgene-mediated CRISPR–Cas reagent delivery methods may be associated with unanticipated genome changes or damage1,2, with prolonged breeding cycles involving foreign DNA segregation and with regulatory restrictions regarding transgenesis3. Therefore, DNA-free delivery has been developed by transfecting preassembled CRISPR–Cas9 ribonucleoproteins into protoplasts4 or in vitro fertilized zygotes5. However, technical difficulties in regeneration from these wall-less cells make impractical a general adaption of these approaches to most crop species. Alternatively, CRISPR–Cas ribonucleoproteins or RNA transcripts have been biolistically bombarded into immature embryo cells or calli to yield highly specific genome editing, albeit at low frequency6–9. Here we report the engineering of a plant negative-strand RNA virus-based vector for DNA-free in planta delivery of the entire CRISPR–Cas9 cassette to achieve single, multiplex mutagenesis and chromosome deletions at high frequency in a model allotetraploid tobacco host. Over 90% of plants regenerated from virus-infected tissues without selection contained targeted mutations, among which up to 57% carried tetra-allelic, inheritable mutations. The viral vector remained stable even after mechanical transmission, and can readily be eliminated from mutated plants during regeneration or after seed setting. Despite high on-target activities, off-target effects, if any, are minimal. Our study provides a convenient, highly efficient and cost-effective approach for CRISPR–Cas9 gene editing in plants through virus infection. A DNA-free in planta approach for gene editing based on RNA virus infection is developed, allowing delivery of the entire CRISPR–Cas9 cassettes into tobacco host to achieve highly efficient single, multiplex mutagenesis and chromosome deletions. |
| Databáze: | OpenAIRE |
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