Pharmacokinetic study on hirsutine and hirsuteine in rats using UPLC–MS/MS
| Autor: | Aixia Han, Jinzhang Cai, Jianshe Ma, Qing Wu, Guanyang Lin, Chongliang Lin, Xianqin Wang, Peiwu Geng |
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| Rok vydání: | 2019 |
| Předmět: |
Chromatography
010405 organic chemistry Formic acid 010401 analytical chemistry Selected reaction monitoring General Chemistry Mass spectrometry 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Bioavailability chemistry.chemical_compound chemistry Pharmacokinetics Hirsuteine Acetonitrile |
| Zdroj: | Acta Chromatographica. 31:99-104 |
| ISSN: | 2083-5736 |
| DOI: | 10.1556/1326.2017.00365 |
| Popis: | An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was established to determine hirsutine and hirsuteine in rat plasma. Pharmacokinetics of hirsutine and hirsuteine in rats after intravenous or oral administration has been investigated using this developed UPLC–MS/MS method, and bioavailability of the two drugs was calculated. Diazepam was used as internal standard, and UPLC BEH column (2.1 mm × 50 mm, 1.7 μm) was used at temperature of 40 °C. The mobile phase was composed of acetonitrile and water (containing 0.1% formic acid) at a gradient elution flow rate of 0.4 mL/min. Nitrogen was used as desolvation gas (800 L/h) and conical gas (50 L/h). The multiple reaction monitoring (MRM) model was applied to quantitatively analyze hirsutine m/z 369 → 226, hirsuteine m/z 367 → 169.9, and diazepam (internal standard) m/z 285.1 → 193.3. Rat plasma samples were deproteinized using acetonitrile prior to UPLC–MS/MS analysis. Within the concentration range of 1–200 ng/mL, the lin... |
| Databáze: | OpenAIRE |
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