Antioxidant responses of Chilo suppressalis (Lepidoptera: Pyralidae) larvae exposed to thermal stress
| Autor: | Ya-Dong Cui, Yu-Zhou Du, Ming-Xing Lu, Cheng-Kui Qiang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Antioxidant biology Physiology medicine.medical_treatment fungi Chilo suppressalis biology.organism_classification medicine.disease_cause Biochemistry Molecular biology Enzyme assay Cell biology Hsp70 Enzyme chemistry Apoptosis medicine biology.protein General Agricultural and Biological Sciences Oxidative stress Developmental Biology |
| Zdroj: | Journal of Thermal Biology. 36:292-297 |
| ISSN: | 0306-4565 |
| Popis: | High temperatures cause a variety of physiological stress responses in insects, including increased generation of reactive oxygen species (ROS), which can cause oxidative damage. This study investigated the effects of thermal stress on ROS generation, the expression of heat shock protein 70 (Hsp70) at the mRNA and protein levels, the activity of antioxidant enzymes (SOD, CAT), and apoptosis in hemocytes of Chilo suppressalis larvae. Results indicated that thermal stress significantly elevated the level of ROS and antioxidant enzyme activity in C. suppressalis larvae. Real-time quantitative PCR showed that hsp70 gene expression was induced by heat stress. Flow cytometric results revealed that the expression profile of Hsp70 at the protein level was in agreement with that at the mRNA level. The expression of Hsp70 at both the mRNA and protein levels reached a maximum at 36 °C in larval hemocytes. Exposure to tested temperatures did not cause any significant change in the rate of apoptosis in larval hemocytes. These results suggest that thermal stress leads to oxidative stress and that antioxidant enzymes and the Hsp70 play an important role in reducing oxidative damage in C. suppressalis larvae. |
| Databáze: | OpenAIRE |
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