Autor: Bruno Zaccomer, Lise Jouanin, Cécile Girard, A. L. Picard-Nizou, Minh-Hà Pham-Delègue, Etienne Grallien
Rok vydání: 1998
Předmět:
Zdroj: Transgenic Research. 7:239-246
ISSN: 0962-8819
DOI: 10.1023/a:1008813910751
Popis: The impact on beneficial insects of proteinase inhibitors expressed in pest-resistant transgenic crops needs to be assessed before the release of these plants into the environment. Three proteinase inhibitors, suitable for incorporation into oilseed rape, were tested on worker bees: the chicken egg white cystatin, oryzacystatin I (OCI) and Bowman-Birk soyabean inhibitor (BBI). Ingestion of low doses of the inhibitors did not cause short-term mortality, and a conditioned proboscis extension assay showed that olfactory learning performances were unchanged when the inhibitors were added to the reward. Long-term ingestion of BBI or OCI did not disrupt total digestive proteolytic activity, but ingestion of BBI induced a new proteinase form, suggesting the existence of a mechanism of control of proteinase synthesis in the honeybee.
Databáze: OpenAIRE