Autor: | Bruno Zaccomer, Lise Jouanin, Cécile Girard, A. L. Picard-Nizou, Minh-Hà Pham-Delègue, Etienne Grallien |
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Rok vydání: | 1998 |
Předmět: |
chemistry.chemical_classification
Proteases Protease medicine.diagnostic_test medicine.medical_treatment Proteolysis fungi Genetically modified crops Biology Protease inhibitor (biology) Microbiology Enzyme chemistry Botany Toxicity Genetics medicine Ingestion Animal Science and Zoology Agronomy and Crop Science Biotechnology medicine.drug |
Zdroj: | Transgenic Research. 7:239-246 |
ISSN: | 0962-8819 |
DOI: | 10.1023/a:1008813910751 |
Popis: | The impact on beneficial insects of proteinase inhibitors expressed in pest-resistant transgenic crops needs to be assessed before the release of these plants into the environment. Three proteinase inhibitors, suitable for incorporation into oilseed rape, were tested on worker bees: the chicken egg white cystatin, oryzacystatin I (OCI) and Bowman-Birk soyabean inhibitor (BBI). Ingestion of low doses of the inhibitors did not cause short-term mortality, and a conditioned proboscis extension assay showed that olfactory learning performances were unchanged when the inhibitors were added to the reward. Long-term ingestion of BBI or OCI did not disrupt total digestive proteolytic activity, but ingestion of BBI induced a new proteinase form, suggesting the existence of a mechanism of control of proteinase synthesis in the honeybee. |
Databáze: | OpenAIRE |
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