| Autor: |
null Shashidhara KS, null Prashanth Kumar HP, null Sameer Srivastava, null Gaikwad SM |
| Rok vydání: |
2012 |
| Zdroj: |
International Journal of Fundamental and Applied Sciences (IJFAS). 1:53-55 |
| ISSN: |
2278-1404 |
| DOI: |
10.59415/ijfas.v1i4.34 |
| Popis: |
BACKGROUD & OBJECTIVE: The α-mannosidase gene from Aspergillus fischeri was isolated by DNA and RNAmethod. METHODOLOGY: Cloning of gene, isolation of recombinant plasmid and sequencing of gDNA gene wascarried out and bioinformatic analysis was done for gene to get intron-exon information and for sequence homology.RESULTS:The α-mannosidase gene from Aspergillus fischeri is 1990 base pair long and sequence analysis showedpresence of three introns and four exons in the gene. BLAST analysis revealed 100% similarity to the Neosartoryafischeri (A. fischeri) and 94% with Aspergillus fumigatus class I α-mannosidase reported in NCBI Gene Data Bank.CONCLUSION: The neutral α-mannosidase from Aspergillus fischeri could belong to the Class IIC subfamily. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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