Gas Chromatographic Separation and Identification of Jacaric and Punicic 2-Ethyl-1-Hexyl Esters

Autor: Kornkanok Aryusuk, Supathra Lilitchan, Narumon Jeyashoke, Kanit Krisnangkura, Siriluck Pojjanapornpun
Rok vydání: 2017
Předmět:
Zdroj: Journal of the American Oil Chemists' Society. 94:511-517
ISSN: 1558-9331
0003-021X
DOI: 10.1007/s11746-017-2965-3
Popis: The present study demonstrates the separation of a critical pair of conjugated linolenic acid (CLN) isomers—jacaric acid (JA; c8, t10, c12-18:3) and punicic acid (PA; c9, t11, c13-18:3)—on a 60-m conventional Supelcowax 10 column. The alkyl esters of different alcohols (C1–C8) of JA and PA were prepared and analyzed isothermally at 220, 230 and 240 °C. The adequacy of their separation was determined from the separation factors (α) and peak resolutions (R s). Acceptable resolution (R s = 1.01) of JA and PA was obtained with their 2-ethyl-1-hexyl ester derivatives at a column temperature of 230 °C. In addition, the Gibbs energy of transfer from solution to gas of the three double bonds $$(\Delta_{\text{sln}}^{\text{g}} G_{\text{u}}$$ ) could be used to describe the interactions of the double bond with the stationary phase. Characterization of 2-ethyl-1-hexyl esters of Jacaranda mimosifolia seed oil at 230 °C demonstrates that the oil contains JA and α- and β-calendic acid as a CLN without the presence of PA. The results suggested that JA could be resolved from PA on a 60-m Supelcowax 10 column as the ethyl hexyl ester.
Databáze: OpenAIRE
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