Characterization of murine lymphocyte activation and exhaustion markers by a 14-color flow cytometry panel

Autor:	Daniel C Rose, Annah S Rolig, William L Redmond
Rok vydání:	2023
Předmět:	Medical Laboratory Technology Clinical Biochemistry General Medicine General Pharmacology Toxicology and Pharmaceutics Analytical Chemistry
Zdroj:	Bioanalysis.
ISSN:	1757-6199 1757-6180
DOI:	10.4155/bio-2023-0005
Popis:	Previously designed flow cytometry panels have provided a framework to analyze T-cell activation; however, few provide an extensive view of lymphocyte populations, and none are optimized for murine models. This article describes a panel designed specifically to assess the expression of activation and exhaustion markers in expanding lymphocyte populations in tumor-bearing mice across two distinct genetic backgrounds: BALB/c and C57BL/6. This comprehensive panel enables the assessment of multiple functional states and immune checkpoint markers across cytotoxic CD8+ T cells, helper and regulatory CD4+ T cells and NK cells in murine whole blood, lymph nodes and tumor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b0c58827ccbef88c2bc59fef5f4b6f43 https://doi.org/10.4155/bio-2023-0005 Zobrazit plný text záznamu