A Comparison of Four Frequently Used Assays for Quantitative Determination of DNA

Autor:	L. C. Gheyssens, M. Van Lancker
Rok vydání:	1986
Předmět:	Biochemistry (medical) Clinical Biochemistry Diphenylamine Biochemistry Quantitative determination Analytical Chemistry Staining chemistry.chemical_compound chemistry Electrochemistry Determination methods DAPI Fluorescent stain Spectroscopy DNA
Zdroj:	Analytical Letters. 19:615-623
ISSN:	1532-236X 0003-2719
DOI:	10.1080/00032718608064524
Popis:	Four frequently used DNA determination methods are compared:the diphenylamine colorimetric assay and three fluorescent stain methods, i.e. diaminobenzoic acid diaminophenylindole and bisimidazole (Hoechst 33258). Interference with these assays by thirty-five substances, on different DNA preparations, are examined. The classical diphenylamine method is found to be the most reliable of all, closely. followed by diaminobenzoic acid staining. The remaining two methods, although very sensitive, perform rather poorly.
Databáze:	OpenAIRE
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