Graphene oxide-based selection and identification of ofloxacin-specific single-stranded DNA aptamers
| Autor: | Yuanding You, Ziwei Xia, Nandi Zhou, Yuhong Zhang, Yaping Tian, Xuyan Han |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Graphene
General Chemical Engineering Aptamer 010401 analytical chemistry Oxide Analytical chemistry 02 engineering and technology General Chemistry DNA Aptamers 021001 nanoscience & nanotechnology 01 natural sciences Combinatorial chemistry 0104 chemical sciences law.invention Dissociation constant chemistry.chemical_compound chemistry law medicine Ofloxacin 0210 nano-technology High homology Systematic evolution of ligands by exponential enrichment medicine.drug |
| Zdroj: | RSC Advances. 6:99540-99545 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c6ra18430h |
| Popis: | Graphene oxide (GO) was introduced in the process of systematic evolution of ligands by exponential enrichment (SELEX) to screen ofloxacin-specific single-stranded DNA aptamers. Four sequences with high homology, ap1, ap3, ap4 and ap5 were picked out. Their secondary structures were simulated, and the dissociation constants (Kd) were measured, which were 251.3, 130.1, 159.1 and 304.4 nM, respectively. The specificity of the aptamers was also evaluated. |
| Databáze: | OpenAIRE |
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