Direct Regulation of the EFR-Dependent Immune Response by Arabidopsis TCP Transcription Factors
| Autor: | Fei Gao, Christopher M. Garner, M. Shahid Mukhtar, Benjamin J. Spears, Walter Gassmann, T C Howton |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Innate immune system biology Physiology fungi Promoter General Medicine biology.organism_classification 01 natural sciences Cell biology 03 medical and health sciences 030104 developmental biology Transcription (biology) Arabidopsis Gene expression Arabidopsis thaliana Agronomy and Crop Science Gene Transcription factor 010606 plant biology & botany |
| Zdroj: | Molecular Plant-Microbe Interactions®. 32:540-549 |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/mpmi-07-18-0201-fi |
| Popis: | One layer of the innate immune system allows plants to recognize pathogen-associated molecular patterns (PAMPS), activating a defense response known as PAMP-triggered immunity (PTI). Maintaining an active immune response, however, comes at the cost of plant growth and development; accordingly, optimization of the balance between defense and development is critical to plant fitness. The TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factor family consists of well-characterized transcriptional regulators of plant development and morphogenesis. The three closely related class I TCP transcription factors TCP8, TCP14, and TCP15 have also been implicated in the regulation of effector-triggered immunity, but there has been no previous characterization of PTI-related phenotypes. To identify TCP targets involved in PTI, we screened a PAMP-induced gene promoter library in a yeast one-hybrid assay and identified interactions of these three TCPs with the EF-Tu RECEPTOR (EFR) promoter. The direct interactions between TCP8 and EFR were confirmed to require an intact TCP binding site in planta. A tcp8 tcp14 tcp15 triple mutant was impaired in EFR-dependent PTI and exhibited reduced levels of PATHOGENESIS-RELATED PROTEIN 2 and induction of EFR expression after elicitation with elf18 but also increased production of reactive oxygen species relative to Col-0. Our data support an increasingly complex role for TCPs at the nexus of plant development and defense. |
| Databáze: | OpenAIRE |
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