Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

Autor:	Anja Nickstadt, Thomas Lahaye, Heidi Scholze, Simone Hahn, Angelika Landgraf, Sabine Kay, Jens Boch, Sebastian Schornack, Ulla Bonas
Rok vydání:	2009
Předmět:	Transcription Activator-Like Effectors Transcription activator-like effector nuclease Multidisciplinary Base pair DNA-binding domain Biology biology.organism_classification DNA-binding protein Cell biology chemistry.chemical_compound TAL effector Xanthomonas chemistry Biochemistry DNA
Zdroj:	Science. 326:1509-1512
ISSN:	1095-9203 0036-8075
DOI:	10.1126/science.1178811
Popis:	TAL Order Xanthomonas bacteria attack their plant hosts by delivering their own transcription-activator–like (TAL) proteins into the plant cell nucleus and alter the plant's gene regulation (see the Perspective by Voytas and Joung ). Moscou and Bogdanove (p. 1501 , published online 29 October: see the cover) and Boch et al. (p. 1509 , published online 29 October) have now discovered how the similar but not identical repeats in the TAL proteins encode the specificity needed for the proteins to find their targets. Each repeat is specific for one DNA base pair, a specificity encoded by hypervariable amino acid positions. Combining several repeats with different amino acids in the hypervariable positions allowed the production of new effectors that targeted new DNA sites.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b073da2bdc786b305da9dc77f249869c https://doi.org/10.1126/science.1178811 Zobrazit plný text záznamu Plný text ve formátu PDF