| Autor: | Moshe White, I-Chun Kuo, Lin-Tang Goh, Kaw Yan Chua, Sa Luo |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Blomia tropicalis
Bioengineering General Medicine Biology equipment and supplies medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Yeast Fed-batch culture Microbiology law.invention Pichia pastoris chemistry.chemical_compound Allergen chemistry law Recombinant DNA medicine Bioreactor Glycerol Food science Biotechnology |
| Zdroj: | Biotechnology Letters. 23:661-665 |
| ISSN: | 0141-5492 |
| DOI: | 10.1023/a:1010323611039 |
| Popis: | The gene of Blomia tropicalis group 5 allergen (Blo t 5) was cloned and expressed in Pichia pastoris KM71. Selected KM71 clones were cultivated in a fed-batch bioreactor feeding first glycerol then followed by methanol. Recombinant Blo t 5 constituted about 30% of the total broth protein after 60 h cultivation. The harvested broth was purified to >95% purity by a two-step anion exchange chromatography. The overall yield was 37 mg Blo t 5 per litre broth. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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