Intraspecific gene expression variability in the yeast revealed by micro-array analysis
| Autor: | Joëlle Reitz-Ausseur, Serge Casaregola, Noémie Jacques, Audrey Suleau |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Kluyveromyces lactis
Regulation of gene expression Genetics 0303 health sciences Fungal protein biology 030306 microbiology General Medicine biology.organism_classification Applied Microbiology and Biotechnology Microbiology Yeast Gene expression profiling 03 medical and health sciences Kluyveromyces Gene expression Gene 030304 developmental biology |
| Zdroj: | FEMS Yeast Research. 5:595-604 |
| ISSN: | 1567-1356 |
| DOI: | 10.1016/j.femsyr.2004.11.009 |
| Popis: | Using the Genolevures sequencing data, we developed an expression micro-array for the yeast Kluyveromyces lactis consisting of 482 genes, mainly involved in central metabolism, compound transport facilitators and stress response. The array was validated using the LAC/GAL system. By comparing gene expression in the laboratory reference strain CBS2359 and in an industrial strain B1, we demonstrated the influence of two carbon sources, glucose and lactose, on the expression of genes involved in the respiratory and in the fermentative metabolic pathways. We also showed that the two strains, although both originating from dairies, display unexpected differences in gene expression on each type of carbon source. |
| Databáze: | OpenAIRE |
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