Isolation and properties of a 23-KD haemolymph protein from the tsetse, Glossina morsitans morsitans

Autor: E. K. Nguu, Ellie O. Osir, James O. Ochanda, Norah K. Olembo
Rok vydání: 1992
Předmět:
Zdroj: International Journal of Tropical Insect Science. 13:189-197
ISSN: 1742-7592
1742-7584
DOI: 10.1017/s174275840001434x
Popis: The haemolymph of the tsetse, Glossina morsitans morsitans, contains a low molecular weight protein of very high density (1.29 g/ml). The protein was detected in the haemolymph during all developmental stages of the insect. Purification of the protein was achieved by a combination of density gradient ultracentrifugation and repeated gel permeation chromatography. Electrophoresis under non-denaturing and denaturing conditions showed the protein to be a single polypeptide chain (M ~23,000). Amino acid analysis revealed a relatively high content of the acidic amino acids as well as serine and glycine. The protein contained lipids as shown by Sudan Black staining but was non-glycosylated. Using rabbit antiserum against the isolated protein in immunodiffusion and immunoblotting experiments, no cross-reactivity was detected with haemolymph samples from insects representing six orders. Although the function of the protein remains unknown, its uniqueness to Glossina suggests that it may have a role in the physiology of this insect.
Databáze: OpenAIRE
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