| Autor: |
Robert L. Dedrick, John K. Hsiao, Paul F. Morrison, Kevin H. Dykstra, Peter M. Bungay, Ivan N. Mefford |
| Rok vydání: |
1991 |
| Předmět: |
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| DOI: |
10.1016/b978-0-444-81194-3.50008-3 |
| Popis: |
Publisher Summary This chapter discusses overall concepts of quantitative microdialysis, including some difficulties of application. The major factors that may influence the proper quantitative use of the microdialysis probe include: (1) microdialysate concentrations obtained in vitro depend strongly on microvascular transport and metabolism in the extracellular space surrounding the probe, as well as diffusion; (2) microdialysis probes may establish concentration gradients about themselves that may be of sufficient extent to engage in mass transfer with anatomical regions that are outside the desired sampling or infusion volume; (3) in in vivo application, the permeability of the common dialysis membranes used in current probes usually plays only a minor role because most of the resistance to mass flow into or out of the probe arises from the tissue and not the membrane; and (4) estimates of extracellular concentration obtained from microdialysis data by the analyses are spatially averaged quantities. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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