Construction of biosensor for determination of galactose with galactose oxidase immobilized on polymeric mediator contains ferrocene
| Autor: | M. Fatih Abasıyanık, Mehmet Şenel, Emre Cevik |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Detection limit
chemistry.chemical_classification Chromatography Chemistry technology industry and agriculture General Physics and Astronomy macromolecular substances Michaelis–Menten kinetics chemistry.chemical_compound Enzyme Ferrocene Galactose oxidase Galactose Organic chemistry General Materials Science Amine gas treating Biosensor |
| Zdroj: | Current Applied Physics. 10:1313-1316 |
| ISSN: | 1567-1739 |
| DOI: | 10.1016/j.cap.2010.03.014 |
| Popis: | A galactose biosensor was developed by immobilizing galactose oxidase (GAox) on poly (glycidyl methacrylate-co-vinylferrocene) (poly (GMA-co-VFc)) film. The enzyme was immobilized via amine group onto polymeric mediator. The biosensor was characterized by the specific activity of the immobilized galactose oxidase, the apparent Michaelis constant K M app , and the stability expressed by time and a number of the performed analysis. The optimized galactose biosensor exhibited a linear response range from 2 to 20 M and detection limit of 0.1 mM toward galactose. The response time of the biosensor was 5 s. The effects of the pH and the temperature of the immobilized galactose oxidase electrode were also studied. |
| Databáze: | OpenAIRE |
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