Capillary Liquid Chromatographic and Automated MALDI-TOF Mass Spectrometric Analysis of Complex Carbohydrate Mixtures

Autor:	and Joanne Charlwood, Rudi Grimm, Helen Birrell, Patrick Camilleri, Gordon A. Ross
Rok vydání:	1998
Předmět:	Complex carbohydrate Analyte Chromatography Capillary action Chemistry Mass spectrometry High-performance liquid chromatography Mass spectrometric Diode array Analytical Chemistry
Zdroj:	Analytical Chemistry. 70:3840-3844
ISSN:	1520-6882 0003-2700
DOI:	10.1021/ac980235h
Popis:	Reproducible microbore reversed-phase HPLC methodology has been developed, suitable for the analysis of carbohydrates derivatized with 2-aminoacridone. The high extinction coefficient of this label at around 260 nm, combined with the use of a new diode array microflow cell, has allowed detection at femtomole levels. Analytes were also robotically collected onto MALDI targets, and mass spectrometric information was acquired for carbohydrate present at low picomole levels. This technology was successfully applied to mixtures of linear and branched oligosaccharides, including sialylated species.
Databáze:	OpenAIRE
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