Regulation of gene expression of hcp, a core gene of the type VI secretion system in Acinetobacter baumannii causing respiratory tract infection
| Autor: | Peng Liu, Ya-dong Zhang, Cai-xia Liu, Yinyin Hu, Xiao-shun Xiong, Xiang-yang Li, Zhuo-ying Wu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Regulation of gene expression biology business.industry 030106 microbiology Respiratory infection General Medicine biology.organism_classification Microbiology Acinetobacter baumannii 03 medical and health sciences medicine.anatomical_structure Gene expression Medicine Respiratory system business Gene Respiratory tract Type VI secretion system |
| Zdroj: | Journal of Medical Microbiology. 67:945-951 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.000753 |
| Popis: | Purpose. The objective of the current study was to investigate whether hcp plays a role in the process of Acinetobacter baumannii infection and to examine clinically relevant factors that may affect hcp expression. Methodology. Seventy-seven A. baumannii isolates from patients with a respiratory infection at the Second Affiliated Hospital and Yuying Childrens Hospital of Wenzhou Medical University (Wenzhou, China) were included in this study. PCR was performed to screen for the presence of hcp. Quantitative real time polymerase chain reaction (qRT-PCR) was carried out to examine the expression of hcp. Results. A total of 77.9 % (60 of 77) of the A. baumannii clinical isolates possessed the hcp gene. Expression of hcp was found to be strain-specific and associated with the infection status. Higher gene expression of hcp was found for invasive A. baumannii isolates causing an infection relative to the colonization group, and for the same strain at a post-infection status compared with that prior to infection. Acid environment was also found to be a trigger of hcp gene expression. Conclusion. The type VI secretion system and hcp predominate in A. baumannii causing respiratory infections. Expression of hcp is regulated by the infection status and acid environment, and might play a role in the process of triggering infection by the colonizer. |
| Databáze: | OpenAIRE |
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